Brant Faircloth
The tables listed below contain published microsatellite primers for Galliformes and all Gallus gallus primers that have been shown to amplify at least one other Galliform species.
Soon we hope to have these tables input to a database that is web accessible through this site (such that the results may be searched by species name, primer sequences, accession numbers, etc.).
Instructions for use:
1.Go to Table 1 and note the numerical code of the species that you are interested in.
2.Go to Table 2 and use the find facility on your browser to search for the numerical code of your chosen species.
3.Click on the links in the last column to get more information. In some instances the name of primer given will differ between publications, the primer sequence rather than its name should be used as a reference point when comparing two studies.
Interpretation:
1.Column 3 is a list of all the species that are known to have been tested with each primer.
2.Column 4 lists all those species that successfully amplified with each primer.
3.Column 5 lists those species that are known to be polymorphic for the marker amplified. [In many cases this has not been determined, as not enough individuals have been amplified – see specific studies for details]
4.Column 6 provides links or references to individual studies in which further information, such as amplification conditions are given.
Unpublished references:
Baker, Unpub. Molecular Markers in Megapodes
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Galliformes Microsatellite Database (Table 1)
A Microsoft Excel table detailing the species in which primers from Table 2 amplify.
Galliformes Microsatellite Database (Table 2)
A tabular compilation (Micrsoft Word format) of microsatellite markers cross-utilized in various species of Galliformes listed in table 1.
Galliformes Microsatellite Database References
References (Microsoft Word Format) for Tables 1 and 2.
Galliformes Microsatellite Dbase (Table 1 - PDF Format)
PDF version of Microsoft Excel Table Above
Galliformes Microsatellite Dbase (Table 2 - PDF Format)
HTML Links to accession numbers DO NOT work in this PDF file.
Galliformes Microsatellite Database References (PDF Format)
Reference list for Tables 1 and 2 in PDF format.
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Databases
NCBI Taxonomy DatabaseNCBI Taxonomy database search for Order Galliformes holdings (listed by genus and genus-specific epithet)
Specialist Groups
Cracid GroupGrouse Group
Megapode Group
Pheasant Group
PQF Group
Other Labs and Protocols
Bruce Roe's Lab ProtocolsVery thorough site from the lab of Bruce Roe
Sheffield Molecular Genetics Facility
The University of Sheffield’s molecular genetics website.
SREL DNA Lab
Travis Glenn’s lab at Savannah River Ecology Lab.
SREL DNA Lab Protocols
Protocols from the Glenn Lab
Tavi's PCR Protocols
Discusses PCR and associated protocols/problems
Suppliers
Applied BiosystemsEquipment, reagents, and primers
Bio-Rad
Equipment, reagents, and consumables
Brinkman-Eppendorf
Equipment, reagents, and consumables
Fisher Scientific
Equipment, reagents, and consumables
IDT DNA
Primers
Marsh Bio-Products
Reagents, consumables
Molecular Probes
Picogreen quantitation reagents
MWG Biotech
Primers, small-scale syntheses
Rainin, Inc.
Pipets and tips
Sigma-Aldrich
Reagents and other chemicals