Publications

Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus).

Schable, N. A., B. C. Faircloth, W. E. Palmer, J. P. Carroll, L. Wes Burger, L. A. Brennan, C. Hagen, and T. C. Glenn. 2004. Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus). Molecular Ecology Notes 4:415-419.
DOI:10.1111/j.1471-8286.2004.00670.x

Abstract

We describe polymerase chain reaction (PCR) primers and conditions to amplify eight dinucleotide, one trinucleotide and 14 tetranucleotide microsatellite DNA loci isolated from the northern bobwhite (Colinus virginianus). The PCR primers were tested on 16 individuals collected from a population located within the Red Hills region of south Georgia and north Florida. The 23 primer pairs developed in this study yielded an average of 6.5 alleles per locus (range 2 to 11), an average observed heterozygosity of 0.47 (range 0.06 to 0.94) and average polymorphic information content of 0.60 (range 0.06 to 0.85).

Microsatellite loci from the bobcat (Lynx rufus).

Faircloth, B. C., T. Valentine, S. H. Eo, A. Reid, T. Terhune, W. E. Palmer, T. C. Glenn, C. J. Nairn, and J. P. Carroll. 2005. Tetranucleotide, Trinucleotide, and Dinucleotide loci from the bobcat. Molecular Ecology Notes 5:387-389.
DOI:10.1111/j.1471-8286.2005.00936.x

Abstract

We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range 4-10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

Tetranucleotide microsatellite loci from Eastern Bluebirds (Sialia sialis)

Faircloth, B. C., G. P. Keller, C. J. Nairn, W. E. Palmer, J. P. Carroll, and P. A. Gowaty. Accepted. Tetranucleotide microsatellite loci from Eastern Bluebirds (Sialia sialis). Molecular Ecology Notes.
DOI:10.1111/j.1471-8286.2006.01408.x

Abstract

We describe primers and polymerase chain reaction (PCR) conditions to amplify 18 tetranucleotide microsatellite DNA loci in Eastern Bluebirds (Sialia sialis). The primers were tested using individuals from 2 study sites in Georgia and South Carolina. Among individuals from the Georgia population (n = 23), the primer pairs developed in this study yielded an average of 6.6 alleles per locus (range 2-12), an average observed heterozygosity of 0.56 (range 0.24 to 0.96), and an average polymorphic information content of 0.65 (range 0.3 to 0.86). Among individuals from the South Carolina population (n = 19), the primer pairs yielded an average of 5.8 alleles per locus (range 2-9), an average observed heterozygosity of 0.56 (range 0.05 to 0.86), and an average polymorphic information content of 0.63 (range 0.29 to 0.83).

GMCONVERT: File conversion for GeneMapper output files

Faircloth, B. C. Accepted. GMCONVERT: File conversion for GeneMapper output files. Molecular Ecology Notes.
DOI: 10.1111/j.1471-8286.2006.01419.x

Abstract

GMCONVERT is a platform independent program provided in GUI (for Apple OS X and Windows XP) and command-line versions (for other platforms). GMCONVERT allows rapid reformatting of microsatellite data from output files produced by Applied Biosystems GeneMapper™ software (v3.x). The program will re-array data into 3 formats commonly used in downstream analysis: GENEPOP, CERVUS, and GERUD. GMCONVERT will greatly increase the speed of data preparation prior to analysis and aid in reducing transpositional errors associated with manual re-arraying and reformatting steps. GMCONVERT is available from http://gallus.forestry.uga.edu/software/.

Publications (in prep)

Tetranucleotide microsatellite loci from the raccoon (Procyon lotor).

Faircloth, B. C., T. Valentine, S. H. Eo, A. Reid, T. Terhune, W. E. Palmer, T. C. Glenn, C. J. Nairn, and J. P. Carroll.

Unpublished Primers/Sequences

Microsatellite loci from the Virginia opossum (Didelphis virginiana).

Faircloth,B. C., B. Shamblin, S. H. Eo, T. M. Terhune, T. Valentine, W. E. Palmer, T. C. Glenn, C. J. Nairn, and J.P. Carroll. 2005. Microsatellite loci from the Virginia opossum (Didelphis virginiana). Genbank Accession Numbers: DQ279219 - DQ279255.

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