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INCLUDED_REGION
format: INCLUDED_REGION
(interval, optional)
A sub-region of the given sequence in which to pick primers. For example, often the first dozen or so bases of a sequence are vector, and should be excluded from consideration. The value for this parameter has the form:
start
,
length
where
start
is the index of the first base to consider, and
length
is the number of subsequent bases in the primer-picking region.
Brant Faircloth
2006-02-15