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INCLUDED_REGION

format: INCLUDED_REGION$=$ (interval, optional)

A sub-region of the given sequence in which to pick primers. For example, often the first dozen or so bases of a sequence are vector, and should be excluded from consideration. The value for this parameter has the form:

$<$start$>$,$<$length$>$

where $<$start$>$ is the index of the first base to consider, and $<$length$>$ is the number of subsequent bases in the primer-picking region.



Brant Faircloth 2006-02-15