PRIMER_FILE_FLAG

format: PRIMER_FILE_FLAG$=$ (boolean, default 0)

If the associated value is non-0, then Primer3 creates two output files for each input SEQUENCE. File $<$sequence_id$>$.for lists all acceptable left primers for $<$sequence_id$>$, and $<$sequence_id$>$.rev lists all acceptable right primers for $<$sequence_id$>$, where $<$sequence_id$>$ is the value of the PRIMER_SEQUENCE_ID tag ( which must be supplied, see ). In addition, if the input tag
PRIMER_PICK_INTERNAL_OLIGO (see ) is non-0, Primer3 produces a file $<$sequence_id$>$.int, which lists all acceptable internal oligos.