PRIMER_INSIDE_PENALTY

format: PRIMER_QUALITY_RANGE_MAX$=$ (int, default 100)

This experimental parameter might not be maintained in this form in the next release. Non-default values valid only for sequences with 0 or 1 target regions. If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times the number of nucleotide positions by which the primer overlaps the (unique) target to get the 'position penalty'. The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.