Up: ``Global'' Input Tags
format: PRIMER_OUTSIDE_PENALTY (float, default 0.0)
This experimental parameter might not be maintained in this form in the next release. Non-default values valid only for sequences with 0 or 1 target regions. If the primer is part of a pair that spans a target and does not overlap the target, then multiply this value times the number of nucleotide positions from the 3' end to the (unique) target to get the 'position penalty'. The effect of this parameter is to allow Primer3 to include nearness to the target as a term in the objective function.